PCR amplification of microdissected wheat chromosome arms in a simple 'single tube' reaction
نویسندگان
چکیده
منابع مشابه
PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.
A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide convenient priming sites for polymerase chain reaction amplification. In this way, as much as 1 microgram of D...
متن کاملComparison of two PCR techniques used in amplification of microdissected plant chromosomes from rice and wheat.
Linker adaptor-mediated PCR (LAM-PCR) and degenerate oligonucleotide primed PCR (DOP-PCR) are major ways to generate chromosome-specific libraries. In this study, these two PCR techniques were used to amplify the microdissected mitotic and meiotic chromosomes from rice and wheat. The results of amplifications were compared, and the advantages and limitations between the two techniques are prese...
متن کاملChromosome painting in plants: in situ hybridization with a DNA probe from a specific microdissected chromosome arm of common wheat.
We report here on the successful painting of a specific plant chromosome within its own genome. Isochromosomes for the long arm of chromosome 5 of the wheat B genome (5BL) were microdissected from first meiotic metaphase spreads of a monoisosomic 5BL line of the common wheat Triticum aestivum cv. Chinese Spring. The dissected isochromosomes were amplified by degenerate oligonucleotide-primed PC...
متن کاملMapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines.
Wheat-barley disomic and ditelosomic chromosome addition lines have been used as genetic tools for a range of applications since their development in the 1980s. In the present study, we used the Affymetrix Barley1 GeneChip for comparative transcript analysis of the barley cultivar Betzes, the wheat cultivar Chinese Spring, and Chinese Spring - Betzes ditelosomic chromosome addition lines to phy...
متن کاملEffect of Lowering PCR Reaction Volumes and Increasing the Speed of DNA Amplification on the Sensitivity of PCR
ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1993
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1993.04050899.x